R17 208.2 (ATCC® TIB-220)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  /  Disease: Erythroleukemia

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Erythroleukemia
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse transferrin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against mouse transferrin receptor
Comments
Animals were immunized with the mouse erythroleukemia line 745.6.
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
This antibody blocks iron uptake and cell proliferation of most murine hematopoietic cell lines and primary cell cultures.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Two to four times weekly
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgM , IgM
Name of Depositor J Lesley
References

Trowbridge IS, et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505

Basic Documentation
References

Trowbridge IS, et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505