WEHI-265.1 (ATCC® TIB-204)

Organism: Mus musculus, mouse  /  Cell Type: monocyte  /  Disease: Abelson murine leukemia virus-induced tumor

Permits and Restrictions

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Organism Mus musculus, mouse
Cell Type monocyte
Product Format frozen
Culture Properties suspension
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Abelson murine leukemia virus-induced tumor
Strain BALB/c
Applications
Derived from an Abelson induced tumor in a BALB/c mouse.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Derived from an Abelson induced tumor in a BALB/c mouse.
Comments
Derived from an Abelson induced tumor in a BALB/c mouse.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 2 x 10 exp5 and 2 x 10 exp6 cells/ml.
Name of Depositor NL Warner
References

. Hybridomas and cellular immortality. New York: Plenum Press; 1983.

Walker EB, et al. Characterization and functional properties of tumor cell lines in accessory cell replacement assays. J. Immunol. 128: 852-859, 1982. PubMed: 7033383

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. Hybridomas and cellular immortality. New York: Plenum Press; 1983.

Walker EB, et al. Characterization and functional properties of tumor cell lines in accessory cell replacement assays. J. Immunol. 128: 852-859, 1982. PubMed: 7033383

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787