2PK-3 (ATCC® TIB-203)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Derivation

Derived from a lymphoma induced with mineral oil and casein in a BALB/c mouse.


Antigen Expression
I-A d; I-E d
Genes Expressed
surface immunoglobulin (sIg +)
Cellular Products
surface immunoglobulin (sIg +)
Comments

It is representative of a mature B lymphocyte just prior to the plasma cell stage of differentiation.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be subcultured by total medium replacement by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Isotype IgG2 kappa , IgG2; kappa light chain
Name of Depositor NL Warner
References

. Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975.

Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906

Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413

Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

Casares S, et al. Antigen presentation of dendritic cells after immunization with DNA encoding a major histocompatability complex class II-restricted viral epitope. J. Exp. Med. 186: 1481-1486, 1997. PubMed: 9348305

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975.

Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906

Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413

Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

Casares S, et al. Antigen presentation of dendritic cells after immunization with DNA encoding a major histocompatability complex class II-restricted viral epitope. J. Exp. Med. 186: 1481-1486, 1997. PubMed: 9348305

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.