EL4.IL-2 (ATCC® TIB-181)

Organism: Mus musculus, mouse  /  Tissue: thymus  /  Disease: lymphoma

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Organism Mus musculus, mouse
Tissue
thymus
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain C57BL/6
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Comments
This is a subline of EL4 (ATCC TIB-39) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA).
The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL. Maintain cell density between 1 to 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor JJ Farrar
References

Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789

Cancer therapeutics. New York: Raven Press; 1981.

Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789

Cancer therapeutics. New York: Raven Press; 1981.

Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931