LBRM-33 clone 4A2 (ATCC® TIB-155)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte; radiation induce  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type T lymphocyte; radiation induce
Product Format frozen
Morphology lymphoblast
Culture Properties suspension; some adherent cells
Biosafety Level 1
Disease lymphoma
Strain B10.BR
Applications
This line was derived from a radiation induced T cell lymphoma.
Production of IL-2 is stimulated by T cell mitogens.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from a radiation induced T cell lymphoma.
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Cellular Products
interleukin-2 (interleukin 2, IL-2)
Comments
This line was derived from a radiation induced T cell lymphoma.
Production of IL-2 is stimulated by T cell mitogens.
Peak levels are found in the culture medium after 24 hours of stimulation with either 1% PHA or 20 microgm/ml Con A.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml Adherent cells can be dislodged by scraping with subsequent resuspension as above.
Cryopreservation
90% FBS + 10% DMSO
Name of Depositor P Ralph
References

Gillis S, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of interleukin-2 producing T cell lymphomas. J. Immunol. 125: 2570-2578, 1980. PubMed: 6968790

Mochizuki D, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma. J. Immunol. 125: 2579-2583, 1980. PubMed: 7000903

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gillis S, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of interleukin-2 producing T cell lymphomas. J. Immunol. 125: 2570-2578, 1980. PubMed: 6968790

Mochizuki D, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma. J. Immunol. 125: 2579-2583, 1980. PubMed: 7000903