IGEL a2 [15.3] (ATCC® TIB-142)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor temperature
Genes Expressed
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Cellular Products
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Comments
Animals were immunized with TNP-Asc.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype IgE (Iga haplotype)
Name of Depositor M Wabl
References

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293

Cross References

Nucleotide (GenBank) : X65774 M.musculus DNA for IgE antibody light chain (VJ).

Nucleotide (GenBank) : X65772 M.musculus DNA for IgE antibody heavy chain (VDJ).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293