CVC.1 (ATCC® TIB-135)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine brain clathrin (light chain, 36000 dalton)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine brain clathrin (light chain, 36000 dalton)
Comments
Animals were immunized with bovine brain clathrin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
    Isotype IgG1
    Name of Depositor FM Brodsky, P Parham
    References

    Kirchhausen T, et al. Location and distribution of the light chains in clathrin trimers. Proc. Natl. Acad. Sci. USA 80: 2481-2485, 1983. PubMed: 6189124

    Brodsky FM, Parham P. Polymorphism in clathrin light chains from different tissues. J. Mol. Biol. 167: 197-204, 1983. PubMed: 6864801

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Kirchhausen T, et al. Location and distribution of the light chains in clathrin trimers. Proc. Natl. Acad. Sci. USA 80: 2481-2485, 1983. PubMed: 6189124

    Brodsky FM, Parham P. Polymorphism in clathrin light chains from different tissues. J. Mol. Biol. 167: 197-204, 1983. PubMed: 6864801