80 V 5B4 (ATCC® TIB-132)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with DEAE purified 79 X1 C2 hybridoma product conjugated to keyhole limpet hemocyanin.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an idiotypic determinant (idiotope, idiotype) on the P3X63Ag8 myeloma protein
Cellular Products
immunoglobulin; monoclonal antibody; against an idiotypic determinant (idiotope, idiotype) on the P3X63Ag8 myeloma protein
Comments
Animals were immunized with DEAE purified 79 X1 C2 hybridoma product conjugated to keyhole limpet hemocyanin.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG2b; kappa light chain
Name of Depositor J Gheuens
References

Gheuens J, McFarlin DE. Use of monoclonal anti-idiotypic antibody to P3/X63/Ag8-myeloma protein for analysis and purification of B-lymphocyte hybridoma products. Eur. J. Immunol. 12: 701-703, 1982. PubMed: 6982818

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gheuens J, McFarlin DE. Use of monoclonal anti-idiotypic antibody to P3/X63/Ag8-myeloma protein for analysis and purification of B-lymphocyte hybridoma products. Eur. J. Immunol. 12: 701-703, 1982. PubMed: 6982818