a Intermediate filament [alpha intermediate filament, anti intermediate filament] (ATCC® TIB-131™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with all classes of intermediate filaments including glial filaments in astrocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against intermediate filaments
Comments
Animals were immunized with human glial fibrillary acidic protein.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with all classes of intermediate filaments including glial filaments in astrocytes.
Neurofilaments in axons.
Tonofilaments in epithelial PtK2 cells and intermediate filaments in fibroblasts.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor R Pruss
References

Pruss RM, et al. All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody. Cell 27: 419-428, 1981. PubMed: 6086105

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Pruss RM, et al. All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody. Cell 27: 419-428, 1981. PubMed: 6086105