MEF (CF-1) IRR (ATCC® SCRC-1040.1)

Organism: Mus musculus, mouse  /  Cell Type: Fibroblast  /  Tissue: Embryo  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue Embryo
Cell Type Fibroblast
Product Format frozen
Morphology Fibroblast
Culture Properties Adherent
Biosafety Level 1
Age 14 days gestation embryo
Gender male and female mixed
Strain CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms
Applications
Irradiated cells for use as feeder layer
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male and female mixed
Comments These cells have been growth-arrested by irradiation with 10,00 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing
    Medium Renewal: Twice a week or when pH decreases
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor ATCC
    Year of Origin 2004
    References

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.