EB1 (ATCC® HTB-60)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Disease Burkitt's lymphoma
Age 9 years
Gender female
Ethnicity Black
Derivation
The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma.
Clinical Data
female
Black
9 years
Comments
The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma.
Viral particles (Epstein-Barr virus) were noted through electron microscopic examination.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isoenzymes
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Name of Depositor G Trempe, LJ Old
References

Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852

Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790

M A Epstein et al. The Behaviour and Morphology of a Second Tissue Culture Strain (EB2) of Lymphoblasts from Burkitt's Lymphoma. Br. J. Cancer 19: 108-115, 1965.

Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014

Epstein MA, et al. Morphological and biological studies on a virus in cultured lymphoblasts from Burkitt's lymphoma. J. Exp. Med. 121: 761-770, 1965.

Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357

Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453

Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. PubMed: 6818551

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852

Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790

M A Epstein et al. The Behaviour and Morphology of a Second Tissue Culture Strain (EB2) of Lymphoblasts from Burkitt's Lymphoma. Br. J. Cancer 19: 108-115, 1965.

Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014

Epstein MA, et al. Morphological and biological studies on a virus in cultured lymphoblasts from Burkitt's lymphoma. J. Exp. Med. 121: 761-770, 1965.

Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357

Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453

Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. PubMed: 6818551