SW 900 [SW-900, SW900] ATCC ® HTB-59™ Homo sapiens lung grad

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	lung
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	grade IV,squamous cell carcinoma
Age	53 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	hypotriploid; modal number = 56. The rate of higher ploidies was 21.8%. Cells in this line have complex karyotypes., Over 30 marker chromosomes were found in 5 metaphase karyotypes. The marker chromosomes, t(1q;10q), t(1q;22q), t(2q;3q), der (3)t(2;3)(p11;q29), der (7)t(7;17) (q36;q21) and about 20 others were common to most cells., Among these der (7) was generally paired. Normal N3, N5, N12, N13, N14 and N17 were absent. The X was single and the Y was not found on fluorescence observation.
Derivation	The SW 900 cell line was initiated by A. Leibovitz in January 1975 from biopsy tissue of a squamous cell carcinoma. The tumor originated in the lung of a 53 year old male Caucasian.
Clinical Data	53 years Caucasian male The tumor originated in the lung of a 53 year old male Caucasian.
Antigen Expression	Antigen expression: Blood type A; Rh+
Tumorigenic	Yes
Comments	The histopathology of the surgical specimen was determined to be grade IV carcinoma.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C Atmosphere: air, 100%

STR Profile	Amelogenin: X CSF1PO: 11 D13S317: 8 D16S539: 11 D5S818: 11 D7S820: 11,12 THO1: 8 TPOX: 11 vWA: 16
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 2

Name of Depositor	A Leibovitz
Passage History	A frozen ampule at passage 15 was received at the ATCC in January, 1982.
Year of Origin	January 1975
References	Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation

Product Sheet

Certificate of Analysis

MSDS

References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

SW 900 [SW-900, SW900] (ATCC® HTB-59™)

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SW 900 [SW-900, SW900] (ATCC^® HTB-59^™)