SW 1088 [SW-1088, SW1088] (ATCC® HTB-12)

Organism: Homo sapiens, human  /  Tissue: brain  /  Disease: astrocytoma

Organism Homo sapiens, human
Tissue brain
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease astrocytoma
Age 72 years
Gender male
Ethnicity Caucasian
Karyotype hypertriploid; modal number = 72 to 74. The rate of higher ploidies was 4.2%. Most chromosomes were morphologically normal. Three marker chromosomes were common to all cells: del(1) (q11), der (9)t(7;?;9) (q11?;?;p24), and der (10)t(4;10) (q21;q15)., The der (9) was paired in nearly 50% of the cells. Usually one, but occasionally three double minutes (DM) were seen in a few cells. Five copies of normal N5, N7 and N20 were seen in most cells., The X and Y were paired. The presence of Y chromosomes was confirmed in the QM stained preparation.
Derivation
The SW 1088 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1975 from an astrocytoma taken from a 72 year old male Caucasian. The cell line was received at the ATCC in January, 1982 at passage 23.
Clinical Data
72 years
Caucasian
male

Antigen Expression
Blood type A; Rh+
Tumorigenic Yes
Effects
Yes, in nude mice
Comments The cells produce a grade III tumor.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 100%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 7,9.3
TPOX: 8
vWA: 16,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 1-2
PGM3, 1
Name of Depositor A Leibovitz
Passage History
The cell line was received at the ATCC in January, 1982 at passage 23.
References

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

Cross References

Nucleotide (GenBank) : AF188519 AF188519 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 1, mRNA sequence.

Nucleotide (GenBank) : AF188520 AF188520 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ASM 3, mRNA sequence.

Nucleotide (GenBank) : AF188521 AF188521 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 2, mRNA sequence.

Nucleotide (GenBank) : AF188522 AF188522 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 3, mRNA sequence.

Nucleotide (GenBank) : AF188523 AF188523 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 4, mRNA sequence.

Nucleotide (GenBank) : AF188524 AF188524 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 5, mRNA sequence.

Nucleotide (GenBank) : AF188525 AF188525 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 1, mRNA sequence.

Nucleotide (GenBank) : AF188526 AF188526 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 2, mRNA sequence.

Nucleotide (GenBank) : AF188527 AF188527 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 3, mRNA sequence.

Nucleotide (GenBank) : AF188528 AF188528 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 4, mRNA sequence.

Nucleotide (GenBank) : AF188529 AF188529 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 5, mRNA sequence.

Basic Documentation
Other Documentation
References

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474