BD5-2d ATCC ® HB-9689™ Mus musculus (B cell); Mus musculus

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Strain	Strain: BALB/c (B cell); BALB/c (myeloma)
Applications	Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein.
Storage Conditions	liquid nitrogen vapor phase

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Derivation	Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells (ATCC CRL 1581). The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids.
Comments	Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells (ATCC CRL 1581). The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing	Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to reach. 1 X 10(6) cells/ml. Interval: Maintain cultures at a cell concentration between 6 x 10(4) and 6 X 10(5) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C

Isotype	mouse IgG1 kappa

Name of Depositor	Applied Biotechnology, Inc
References	Carney WP, McKenzie SJ. Detection and quantification of neu related proteins in the biological fluids of humans. US Patent 5,401,638 dated Mar 28 1995 Carney WP, et al. Detection of neu p185 in cell lysates. US Patent 5,604,107 dated Feb 18 1997 McKenzie SJ, et al. Generation and characterization of monoclonal antibodies specific for the human neu oncogene product, p185. Oncogene 4: 543-548, 1989. PubMed: 2566965 Animals were immunized with NIH/3T3 cells expressing full-length human HER-2/neu protein. Weinberg RA, et al. Oncogenes and methods for their detection. U.S. Patent 6,713,619 dated Mar 30 2004

Name of Depositor

Applied Biotechnology, Inc

References

Carney WP, McKenzie SJ. Detection and quantification of neu related proteins in the biological fluids of humans. US Patent 5,401,638 dated Mar 28 1995

Carney WP, et al. Detection of neu p185 in cell lysates. US Patent 5,604,107 dated Feb 18 1997

McKenzie SJ, et al. Generation and characterization of monoclonal antibodies specific for the human neu oncogene product, p185. Oncogene 4: 543-548, 1989. PubMed: 2566965

Animals were immunized with NIH/3T3 cells expressing full-length human HER-2/neu protein.

Weinberg RA, et al. Oncogenes and methods for their detection. U.S. Patent 6,713,619 dated Mar 30 2004

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph
References	Carney WP, McKenzie SJ. Detection and quantification of neu related proteins in the biological fluids of humans. US Patent 5,401,638 dated Mar 28 1995 Carney WP, et al. Detection of neu p185 in cell lysates. US Patent 5,604,107 dated Feb 18 1997 McKenzie SJ, et al. Generation and characterization of monoclonal antibodies specific for the human neu oncogene product, p185. Oncogene 4: 543-548, 1989. PubMed: 2566965 Animals were immunized with NIH/3T3 cells expressing full-length human HER-2/neu protein. Weinberg RA, et al. Oncogenes and methods for their detection. U.S. Patent 6,713,619 dated Mar 30 2004

BD5-2d (ATCC® HB-9689™)

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BD5-2d (ATCC^® HB-9689^™)