BVD2-21C11.3 (ATCC® HB-9569)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  Lewis (B cell); BALB/c (myeloma)

Applications
The antibody binds to and blocks the activity of human GM-CSF.
Derivation
Animals were immunized with purified human GM-CSF.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human granulocyte - macrophage colony stimulating factor (GM-CSF)
Cellular Products
immunoglobulin; monoclonal antibody; against human granulocyte - macrophage colony stimulating factor (GM-CSF)
Comments
Animals were immunized with purified human GM-CSF.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody binds to and blocks the activity of human GM-CSF.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%.
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor Schering Corp.
References

Abrams JS, VanDyke RE. Immunochemical assay for human granulocyte-macrophage colony stimulating factor. US Patent 5,070,013 dated Dec 3 1991

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Abrams JS, VanDyke RE. Immunochemical assay for human granulocyte-macrophage colony stimulating factor. US Patent 5,070,013 dated Dec 3 1991