Mus musculus (B cell); Mus musculus (myeloma) (ATCC® HB-8963)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Subcultivation
Medium Renewal: Every 2 to 3 days
Agent 2B5
Strain Designations 2B5
Morphology lymphoblast
Applications
It does not neutralize the coagulant activity of HMWK.
immunoglobulin; monoclonal antibody; against human kininogen heavy chain
Application
It does not neutralize the coagulant activity of HMWK.
immunoglobulin; monoclonal antibody; against human kininogen heavy chain
Type Strain no
Biosafety Level 1
Comments
Animals were immunized with purified high molecular weight kininogen (HMWK) isolated from fresh human plasma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the heavy chain (64000 dalton kallikrein cleavage fragment) of human kininogen.
It does not neutralize the coagulant activity of HMWK.
The affinity (apparent Kd) of 2B5 for HMWK was calculated to be 1.91 nM.
suspension
immunoglobulin; monoclonal antibody; against human kininogen heavy chain
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Product Format frozen
Designation / Description 2B5 MOUSE HYBRIDOMA
U.S. Patent Number
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation