WBC264-9C (ATCC® HB-8902)

Organism: human (leukocyte); mouse (macrophage)  /  Cell Type: Macrophage,Macrophage-like  / 

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Organism human (leukocyte); mouse (macrophage)
Cell Type Macrophage,Macrophage-like
Product Format frozen
Morphology macrophage
Culture Properties adherent
Biosafety Level 1
Strain

Strain:  BALB/c (macrophage)

Applications
This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264.
The cells exhibit chemotaxis to N-formyl-methionine-leucine-phenylalanine (fMet-Leu-Phe, FMLP).
Storage Conditions liquid nitrogen vapor temperature
Karyotype at passage 6, the mean number of chromosome was 64 with a range of 36 to 81
Derivation
This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264.
Comments
This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264.
The cells exhibit chemotaxis to N-formyl-methionine-leucine-phenylalanine (fMet-Leu-Phe, FMLP).
WBC264-9 cells express human HPRT.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Subcultures are prepared by scraping. DO NOT USE TRYPSIN! Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor The United States of America
References

Aksamit RR. Human-mouse hybrid cell line expressing monocyte-macrophage properties. US Patent 4,970,162 dated Nov 13 1990

Aksamit RR. A human-mouse hybrid cell line that stably expresses chemotaxis to N- formylmethionyl-leucyl-phenylalanine. Biochem. Biophys. Res. Commun. 138: 1001-1008, 1986. PubMed: 3741419

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Aksamit RR. Human-mouse hybrid cell line expressing monocyte-macrophage properties. US Patent 4,970,162 dated Nov 13 1990

Aksamit RR. A human-mouse hybrid cell line that stably expresses chemotaxis to N- formylmethionyl-leucyl-phenylalanine. Biochem. Biophys. Res. Commun. 138: 1001-1008, 1986. PubMed: 3741419