FHCR-1-2075/FH4 [FH4] (HB-8775)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for di- and tri- fucosylated type 2 chain glycolipids, but does not react with monofucosylated type 2 chains.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against di- and trifucosylated type 2 chain glycolipids
Cellular Products
immunoglobulin; monoclonal antibody; against di- and trifucosylated type 2 chain glycolipids
Comments
Animals were immunized with glycolipids adsorbed to Salmonella minnesota.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody is specific for di- and tri- fucosylated type 2 chain glycolipids, but does not react with monofucosylated type 2 chains.
A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (see ATCC 56-X) is necessary for successful initiation of a culture from a thawed ampule.
Complete Growth Medium RPMI 1640 medium, 85%; fetal bovine serum, 15% - OR - Modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Complete Growth Medium
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml. The line is best carried as an ascites in pristane primed BALB/c mice.
Isotype IgG3
Name of Depositor Fred Hutchinson Cancer Res. Cntr.
U.S. Patent Number
References

Hakomori SI. Hybridomas producing monoclonal antibodies to mono-, di-, and trifucosylated type 2 chain. US Patent 4,876,199 dated Oct 24 1989

Ochi T, et al. The presence of a myeloid cell population showing strong reactivity with monoclonal antibody directed to difucosyl type 2 chain in epiphyseal bone marrow adjacent to joints affected with rheumatoid arthritis (RA) and its absence in the corresponding normal and non-RA bone marrow. J. Rheumatol. 15: 1609-1615, 1988. PubMed: 3236296

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hakomori SI. Hybridomas producing monoclonal antibodies to mono-, di-, and trifucosylated type 2 chain. US Patent 4,876,199 dated Oct 24 1989

Ochi T, et al. The presence of a myeloid cell population showing strong reactivity with monoclonal antibody directed to difucosyl type 2 chain in epiphyseal bone marrow adjacent to joints affected with rheumatoid arthritis (RA) and its absence in the corresponding normal and non-RA bone marrow. J. Rheumatol. 15: 1609-1615, 1988. PubMed: 3236296