FHCR-1-2075/FH4 [FH4] (ATCC® HB-8775)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for di- and tri- fucosylated type 2 chain glycolipids, but does not react with monofucosylated type 2 chains.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against di- and trifucosylated type 2 chain glycolipids
Cellular Products
immunoglobulin; monoclonal antibody; against di- and trifucosylated type 2 chain glycolipids
Comments
Animals were immunized with glycolipids adsorbed to Salmonella minnesota.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody is specific for di- and tri- fucosylated type 2 chain glycolipids, but does not react with monofucosylated type 2 chains.
A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (see ATCC 56-X) is necessary for successful initiation of a culture from a thawed ampule.
Complete Growth Medium RPMI 1640 medium, 85%; fetal bovine serum, 15% - OR - Modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Complete Growth Medium
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml. The line is best carried as an ascites in pristane primed BALB/c mice.
Isotype IgG3
Name of Depositor Fred Hutchinson Cancer Res. Cntr.
References

Hakomori SI. Hybridomas producing monoclonal antibodies to mono-, di-, and trifucosylated type 2 chain. US Patent 4,876,199 dated Oct 24 1989

Ochi T, et al. The presence of a myeloid cell population showing strong reactivity with monoclonal antibody directed to difucosyl type 2 chain in epiphyseal bone marrow adjacent to joints affected with rheumatoid arthritis (RA) and its absence in the corresponding normal and non-RA bone marrow. J. Rheumatol. 15: 1609-1615, 1988. PubMed: 3236296

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hakomori SI. Hybridomas producing monoclonal antibodies to mono-, di-, and trifucosylated type 2 chain. US Patent 4,876,199 dated Oct 24 1989

Ochi T, et al. The presence of a myeloid cell population showing strong reactivity with monoclonal antibody directed to difucosyl type 2 chain in epiphyseal bone marrow adjacent to joints affected with rheumatoid arthritis (RA) and its absence in the corresponding normal and non-RA bone marrow. J. Rheumatol. 15: 1609-1615, 1988. PubMed: 3236296