HM2 (ATCC® HB-8587)

Organism: human (T cell); human (T cell leukemia)  /  Cell Type: hybridoma: helper T lymphocyte  /  Disease: Leukemia,hepatitis

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Organism human (T cell); human (T cell leukemia)
Cell Type hybridoma: helper T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain RETROVIRUS
Disease Leukemia,hepatitis
Applications
The cells produce a SSF that specifically inhibits T cell dependent immune functions.
NOTE: this line should be handled with care since one of the fusion partners was derived from a patient with AIDS.
Hepatitis B virus precautions are recommended by the depositor.
Derivation
T cells from a patient with acquired immunodeficiency syndrome (AIDS) were fused with K37.3.2 human T cell leukemia cells.
NOTE: this line should be handled with care since one of the fusion partners was derived from a patient with AIDS.
Clinical Data
T cells from a patient with acquired immunodeficiency syndrome (AIDS) were fused with K37.3.2 human T cell leukemia cells.
NOTE: this line should be handled with care since one of the fusion partners was derived from a patient with AIDS.
Antigen Expression
Leu 3a; CD4
Genes Expressed
soluble suppression factor (SSF),Leu 3a; CD4
Comments
T cells from a patient with acquired immunodeficiency syndrome (AIDS) were fused with K37.3.2 human T cell leukemia cells.
The cells produce a SSF that specifically inhibits T cell dependent immune functions.
NOTE: this line should be handled with care since one of the fusion partners was derived from a patient with AIDS.
Hepatitis B virus precautions are recommended by the depositor.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture medium: RPMI 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor Cornell Res. Fndn., Inc.
References

Laurence JC. Human T cell hybridomas which produce immunosuppressive factors. US Patent 4,665,032 dated May 12 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Laurence JC. Human T cell hybridomas which produce immunosuppressive factors. US Patent 4,665,032 dated May 12 1987