BTV10XSp2/0-Ag14-10D4.90 (ATCC® HB-8377)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Biosafety Level 1
Age adult
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with a viral protein associated with the replication BTV.
It can be used in immunofluorescence assays to identify the U.S. serotypes of BTV, and differentiate between BTV and the closely related virus, epizootic hemorrhagic disease virus (EHDV).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Bluetongue virus (BTV, orbivirus)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with serotype 10 BTV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a viral protein associated with the replication BTV.
It can be used in immunofluorescence assays to identify the U.S. serotypes of BTV, and differentiate between BTV and the closely related virus, epizootic hemorrhagic disease virus (EHDV).
The immunofluorescence pattern is characterized as abundant discrete granules and large and small spherical inclusions in the cytoplasm of infected cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture medium: HB101, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a kappa
Name of Depositor The United States of America
References

Jochim MM, Jones SC. Monoclonal antibodies to bluetongue virus antigen. US Patent 4,873,189 dated Oct 10 1989

. . Proc. Am. Assoc. Vet. Lab. Diag. : 277-283, 1983.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Jochim MM, Jones SC. Monoclonal antibodies to bluetongue virus antigen. US Patent 4,873,189 dated Oct 10 1989

. . Proc. Am. Assoc. Vet. Lab. Diag. : 277-283, 1983.