5C9 (ATCC® HB-8371)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against porcine (pig) IgM (mu heavy chain)
Cellular Products
immunoglobulin; monoclonal antibody; against porcine (pig) IgM (mu heavy chain)
Comments
Animals were immunized with porcine IgM.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; agamma horse serum, 5%; bovine calf serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor The United States of America
References

Paul PS, Van Deusen RA. Monoclonal antibodies to porcine immunoglobulins. US Patent 4,468,346 dated Aug 28 1984

Burke DS, et al. Japanese encephalitis virus immunoglobulin M antibodies in porcine sera. Am. J. Vet. Res. 46: 2054-2057, 1985. PubMed: 2998237

Basic Documentation
References

Paul PS, Van Deusen RA. Monoclonal antibodies to porcine immunoglobulins. US Patent 4,468,346 dated Aug 28 1984

Burke DS, et al. Japanese encephalitis virus immunoglobulin M antibodies in porcine sera. Am. J. Vet. Res. 46: 2054-2057, 1985. PubMed: 2998237