130-3-F7-5 [CO-301, CO301] (ATCC® HB-8326)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Genes Expressed
immunoglobulin; monoclonal antibody; against human Lewis b blood group antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human Lewis b blood group antigen
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum,
20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor M Herlyn
References

Blaszczyk M, et al. Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch. Biochem. Biophys. 233: 161-168, 1984. PubMed: 6465894

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Blaszczyk M, et al. Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch. Biochem. Biophys. 233: 161-168, 1984. PubMed: 6465894