2T8-3E10 (ATCC® HB-8213)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell antigen (Ti1b antigen) expressed only on the CT8III cytotoxic T lymphocyte clone
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/cJ mice
Comments
Animals were immunized with CT8III cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not react with human thymocytes or resting or activated peripheral T cells.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 64%; NCTC 109 medium, 10%; L-glutamine, 2%; sodium pyruvate, 1%; hypoxanthine-thymidine, 1%; oxaloacetic acid and insulin, 1%; horse serum, 10%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below) with HT, 80%; fetal
bovine serum, 10%; horse serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Horse Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Dana Farber Cancer Inst.
References

Schlossman SF, et al. Monoclonal antibodies that recognize human T cells. US Patent 4,550,086 dated Oct 29 1985

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Schlossman SF, et al. Monoclonal antibodies that recognize human T cells. US Patent 4,550,086 dated Oct 29 1985