2G8.D6 (ATCC® HB-8190)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate)
Cellular Products
immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with p-azobenzylphosphonate covalently linked to a carrier protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor Massachusetts Institute of Technology
References

Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985

Basic Documentation
References

Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985