Designation / Description 1A8 MOUSE T CELL HYBRIDOMA
U.S. Patent Number
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

BW5147 T cell lymphoma cells were fused with phytohemagglutinin stimulated LBRM-33 lymphoma cells.
This line is the parent of 1A8C3C10 (see ATCC HB-8089).
The cells constitutively produce IL-2 and do not require lectin stimulation.
murine interleukin 2 (interleukin-2, IL-2)
RPMI 1640 medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 6 X 10 exp5 cells/ml.
Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation

Stull D, Gillis S. Constitutive production of interleukin-2 activity by a T cell hybridoma. J. Immunol. 126: 1680-1683, 1981. PubMed: 6971310

Gillis S. Constitutive production of interleukin 2 by a T cell hybridoma. US Patent 4,473,642 dated Sep 25 1984