1A8 (ATCC® HB-8090)

Organism: Mus musculus, mouse  / 

Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Biosafety Level 2
Applications
The cells constitutively produce IL-2 and do not require lectin stimulation.
Comments
BW5147 T cell lymphoma cells were fused with phytohemagglutinin stimulated LBRM-33 lymphoma cells.
This line is the parent of 1A8C3C10 (see ATCC HB-8089).
The cells constitutively produce IL-2 and do not require lectin stimulation.
suspension
murine interleukin 2 (interleukin-2, IL-2)
RPMI 1640 medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 6 X 10 exp5 cells/ml.
Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Complete Growth Medium Click's medium, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Click's medium, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Medium Renewal: Every 2 to 3 days
Name of Depositor Immunex Corporation
References

Stull D, Gillis S. Constitutive production of interleukin-2 activity by a T cell hybridoma. J. Immunol. 126: 1680-1683, 1981. PubMed: 6971310

Gillis S. Constitutive production of interleukin 2 by a T cell hybridoma. US Patent 4,473,642 dated Sep 25 1984

Basic Documentation
References

Stull D, Gillis S. Constitutive production of interleukin-2 activity by a T cell hybridoma. J. Immunol. 126: 1680-1683, 1981. PubMed: 6971310

Gillis S. Constitutive production of interleukin 2 by a T cell hybridoma. US Patent 4,473,642 dated Sep 25 1984