34-4-21S (ATCC® HB-76)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with cells from strains that have the H-2d haplotype either in the H-2D region or in the entire major histocompatibility complex.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 D d; cytotoxic
Comments
Animals were immunized with spleen cells from BDF1.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with cells from strains that have the H-2d haplotype either in the H-2D region or in the entire major histocompatibility complex.
A 20 fold concentration of supernatant may be necessary to detect the antibody by immunodiffusion.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Complete Growth Medium
    Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
    serum, 10%.
    DME w/HEPES (10mM) 100 mL
    L-Glutamine (100x) 1 mL
    Solution I 1 mL
    Non-essential amino acids (100x) 1 mL
    NCTC 135 10 mL
    Fetal Bovine Serum 12 mL
    Solution I (100x)
    1. 1320 mg oxalacetic acid (100 mM, MW 132).
    2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
    Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
    100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
    aliquot and store frozen.
    Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype IgM
    Name of Depositor DH Sachs, K Ozato
    References

    Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation 34: 113-118, 1982. PubMed: 7135466

    Basic Documentation
    References

    Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation 34: 113-118, 1982. PubMed: 7135466