MA2.1 (ATCC® HB-54)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA A2, B17
Cellular Products
immunoglobulin; monoclonal antibody; against HLA A2, B17
Comments
Animals were immunized with papain solubilized HLA A2 and B17 antigens from the J-Y cell line.
Spleen cells were fused with NS-1 myeloma cells.
Mutation at positions 62 to 66 of the alpha 1 domain of the HLA-A2 molecule results in complete loss of binding of MA2.1.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Complete Growth Medium
Culture Medium: RPMI medium 1640, 80%; fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor P Parham
References

McMichael AJ, et al. A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17. Hum. Immunol. 1: 121-129, 1980. PubMed: 6167544

Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

Basic Documentation
References

McMichael AJ, et al. A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17. Hum. Immunol. 1: 121-129, 1980. PubMed: 6167544

Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666