CC21 (ATCC® HB-288)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for a 145000 dalton antigen on bovine B cells.
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
The antibody reacts with rat type 1 myosin heavy chains, and binds to slow type I fibres in rat muscle.
immunoglobulin; monoclonal antibody; against WC3 bovine B cell antigen (BoWC3),Expression of WC3 correlates with IgM expression on circulating B cells.
Comments
Animals were immunized with bovine alveolar macrophages.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for a 145000 dalton antigen on bovine B cells.
Expression of WC3 correlates with IgM expression on circulating B cells.
The antigen is also expressed on dendritic cells in lymphoid tissues.
It appears to be the analogue to human CD21.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor CJ Howard
References

Naessens J, et al. Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes. Immunology 69: 525-530, 1990. PubMed: 2185984

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Naessens J, et al. Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes. Immunology 69: 525-530, 1990. PubMed: 2185984