BF-45 (ATCC® HB-278)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with embryonic rat myosin heavy chain, and stains fetal muscle fibres in immunofluorescence assays.
When used at high concentration, some cross-reactivity with adult muscle may be seen.
Derivation
Spleen cells were fused with NSO myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against embryonic myosin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against embryonic myosin heavy chain
Comments
Animals were immunized with bovine fetal skeletal muscle myosin.
Spleen cells were fused with NSO myeloma cells.
The antibody reacts with embryonic rat myosin heavy chain, and stains fetal muscle fibres in immunofluorescence assays.
When used at high concentration, some cross-reactivity with adult muscle may be seen.
The cells were deposited on behalf of Dr. Schiaffino by Regeneron Pharmaceuticals, Inc.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor S Schiaffino
References

Schiaffino S, et al. Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle. Dev. Biol. 127: 1-11, 1988. PubMed: 3282936

Gorza L, et al. An embryonic-like myosin heavy chain is transiently expressed in nodal conduction tissue of the rat heart. J. Mol. Cell. Cardiol. 20: 931-941, 1988. PubMed: 3216402

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Schiaffino S, et al. Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle. Dev. Biol. 127: 1-11, 1988. PubMed: 3282936

Gorza L, et al. An embryonic-like myosin heavy chain is transiently expressed in nodal conduction tissue of the rat heart. J. Mol. Cell. Cardiol. 20: 931-941, 1988. PubMed: 3216402