AP-3 (ATCC® HB-242)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts specifically with GPIIIa, but does not inhibit platelet aggregation.
It is useful for studies of human GPIIIa expression by flow cytometry, Western blot (non-reduced) and affinity chromatography methods.
The antibody can also be used for the classification of human megakaryocytic leukemias and Glanzmann thrombasthenia.
Derivation
Animals were immunized with partially purified human platelet glycoproteins IIb and IIIa (GPIIb and GPIIIa, respectively).
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against beta 3 integrin (platelet GPIIIa)
Cellular Products
immunoglobulin; monoclonal antibody; against beta 3 integrin (platelet GPIIIa)
Comments
Animals were immunized with partially purified human platelet glycoproteins IIb and IIIa (GPIIb and GPIIIa, respectively).
Spleen cells were fused with NS-1 myeloma cells.
The antibody recognizes all known allelic forms of the beta 3 subunit of the integrin superfamily.
The two glycoproteins (GPIIb and GPIIIa) together form the platelet binding site for fibrinogen.
The antibody reacts specifically with GPIIIa, but does not inhibit platelet aggregation.
It is useful for studies of human GPIIIa expression by flow cytometry, Western blot (non-reduced) and affinity chromatography methods.
The antibody can also be used for the classification of human megakaryocytic leukemias and Glanzmann thrombasthenia.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor PJ Newman
References

Newman PJ, et al. Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3. Blood 65: 227-232, 1985. PubMed: 3155488

Newman PJ, et al. Synergistic action of two murine monoclonal antibodies that inhibit ADP- induced platelet aggregation without blocking fibrinogen binding. Blood 69: 668-676, 1987. PubMed: 3026525

Isenberg WM, et al. Monoclonal antibodies bound to subunits of the integrin GPIIb-IIIa are internalized and interfere with filopodia formation and platelet aggregation. Blood 76: 1564-1571, 1990. PubMed: 2207330

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Newman PJ, et al. Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3. Blood 65: 227-232, 1985. PubMed: 3155488

Newman PJ, et al. Synergistic action of two murine monoclonal antibodies that inhibit ADP- induced platelet aggregation without blocking fibrinogen binding. Blood 69: 668-676, 1987. PubMed: 3026525

Isenberg WM, et al. Monoclonal antibodies bound to subunits of the integrin GPIIb-IIIa are internalized and interfere with filopodia formation and platelet aggregation. Blood 76: 1564-1571, 1990. PubMed: 2207330