162-21.2 (ATCC® HB-241)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Choriocarcinoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Choriocarcinoma
Applications
The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas.
The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors.
No staining was detected on peripheral blood cells.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Trop-1 antigen
Comments
Animals were immunized with the human choriocarcinoma cell line BeWo.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas.
The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors.
The gene encoding the trop-1 antigen has been cloned.
No staining was detected on peripheral blood cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor LA Herzenberg
References

Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529

Strnad J, et al. Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA. Cancer Res. 49: 314-317, 1989. PubMed: 2463074

Klein CE, et al. Expression of 38-kD cell-surface glycoprotein in transformed human keratinocyte cell lines, basal cell carcinomas, and epithelial germs. J. Invest. Dermatol. 95: 74-82, 1990. PubMed: 2366004

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529

Strnad J, et al. Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA. Cancer Res. 49: 314-317, 1989. PubMed: 2463074

Klein CE, et al. Expression of 38-kD cell-surface glycoprotein in transformed human keratinocyte cell lines, basal cell carcinomas, and epithelial germs. J. Invest. Dermatol. 95: 74-82, 1990. PubMed: 2366004