4D11 (ATCC® HB-240)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells.
This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells.
The antigen recognized by 4D11 appears to be nonpolymorphic, and is present in all mouse strains tested.
Derivation
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an antigen on mouse NK cells (LGL-1)
Cellular Products
immunoglobulin; monoclonal antibody; against an antigen on mouse NK cells (LGL-1)
Comments
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody detects a major (approximately 50%) subset of mouse NK cells.
This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells.
The antigen recognized by 4D11 appears to be nonpolymorphic, and is present in all mouse strains tested.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor LH Mason
References

Mason LH, et al. Natural killer (NK) cell subsets in the mouse. NK-1.1+/LGL-1+ cells restricted to lysing NK targets, whereas NK-1.1+/LGL-1- cells generate lymphokine-activated killer cells. J. Immunol. 145: 751-759, 1990. PubMed: 2365999

Mason L, et al. LGL-1: a non-polymorphic antigen expressed on a major population of mouse natural killer cells. J. Immunol. 140: 4403-4412, 1988. PubMed: 2453582

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mason LH, et al. Natural killer (NK) cell subsets in the mouse. NK-1.1+/LGL-1+ cells restricted to lysing NK targets, whereas NK-1.1+/LGL-1- cells generate lymphokine-activated killer cells. J. Immunol. 145: 751-759, 1990. PubMed: 2365999

Mason L, et al. LGL-1: a non-polymorphic antigen expressed on a major population of mouse natural killer cells. J. Immunol. 140: 4403-4412, 1988. PubMed: 2453582