12.1 (ATCC® HB-228)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: sarcoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease sarcoma
Applications
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells.
Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts.
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Clinical Data
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD6
Cellular Products
immunoglobulin; monoclonal antibody; against human CD6
Comments
Animals were immunized with human lymphoblasts from a mixed lymphocyte culture reaction.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells.
Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts.
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate,
and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor PJ Martin
References

Kamoun M, et al. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987-991, 1981. PubMed: 6790625

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kamoun M, et al. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987-991, 1981. PubMed: 6790625