12.1 (ATCC® HB-228)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: sarcoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease sarcoma
Applications
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells.
Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts.
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Clinical Data
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD6
Cellular Products
immunoglobulin; monoclonal antibody; against human CD6
Comments
Animals were immunized with human lymphoblasts from a mixed lymphocyte culture reaction.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells.
Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts.
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate,
and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor PJ Martin
References

Kamoun M, et al. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987-991, 1981. PubMed: 6790625

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kamoun M, et al. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987-991, 1981. PubMed: 6790625