2E6 (ATCC® HB-226)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  / 

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes.
Storage Conditions liquid nitrogen vapor temperature
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18)
Comments
Animals were immunized with mouse dendritic cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes.
2E6 blocks the binding function of LFA-1 (CD11a) and CR3/Mac-1 (CD11b).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype IgG
Name of Depositor RM Steinman
References

Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332