IV.3 (ATCC® HB-217)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with a low affinity receptor for the Fc of IgG that is expressed on human platelets, monocytes and granulocytes.
Storage Conditions liqid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a 40000 dalton low affinity IgG Fc receptor on human monocytes, granulocytes and platelets; against CD32
Cellular Products
immunoglobulin; monoclonal antibody; against a 40000 dalton low affinity IgG Fc receptor on human monocytes, granulocytes and platelets; against CD32
Comments
Animals were immunized with K-562 cells (see ATCC CCL-243).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a low affinity receptor for the Fc of IgG that is expressed on human platelets, monocytes and granulocytes.
The receptor is a sialoglycoprotein and appears to be the human equivalent of the mouse Fc gamma receptor recognized by the 2.4G2 monoclonal antibody (ATCC HB-197).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor Medarex, Inc.
References

Looney RJ, et al. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J. Immunol. 136: 1641-1647, 1986. PubMed: 2936805

Anderson CL, et al. Structural polymorphism of the human monocyte 40 kilodalton Fc receptor for IgG. J. Immunol. 138: 2254-2256, 1987. PubMed: 2951436

Rosenfeld SI, et al. Human Fcgamma receptors: stable inter-donor variation in quantitative expression on platelets correlates with functional responses. J. Immunol. 138: 2869-2873, 1987. PubMed: 2952726

Graziano RF, Fanger MW. FcgammaRI and FcgammaRII on monocytes and granulocytes are cytotoxic trigger molecules for tumor cells. J. Immunol. 139: 3536-3541, 1987. PubMed: 2960735

Petroni KC, et al. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. J. Immunol. 140: 3467-3472, 1988. PubMed: 2966197

Graziano RF, et al. FcgammaR-mediated killing by eosinophils. J. Immunol. 142: 230-235, 1989. PubMed: 2521234

. . Immunol. Today 7: 264-266, 1986.

Looney RJ, et al. Structural polymorphism of the human platelet Fc gamma receptor. J. Immunol. 141: 2680-2683, 1988. PubMed: 2971728

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Looney RJ, et al. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J. Immunol. 136: 1641-1647, 1986. PubMed: 2936805

Anderson CL, et al. Structural polymorphism of the human monocyte 40 kilodalton Fc receptor for IgG. J. Immunol. 138: 2254-2256, 1987. PubMed: 2951436

Rosenfeld SI, et al. Human Fcgamma receptors: stable inter-donor variation in quantitative expression on platelets correlates with functional responses. J. Immunol. 138: 2869-2873, 1987. PubMed: 2952726

Graziano RF, Fanger MW. FcgammaRI and FcgammaRII on monocytes and granulocytes are cytotoxic trigger molecules for tumor cells. J. Immunol. 139: 3536-3541, 1987. PubMed: 2960735

Petroni KC, et al. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. J. Immunol. 140: 3467-3472, 1988. PubMed: 2966197

Graziano RF, et al. FcgammaR-mediated killing by eosinophils. J. Immunol. 142: 230-235, 1989. PubMed: 2521234

. . Immunol. Today 7: 264-266, 1986.

Looney RJ, et al. Structural polymorphism of the human platelet Fc gamma receptor. J. Immunol. 141: 2680-2683, 1988. PubMed: 2971728