LM2/1.6.11 (ATCC® HB-204)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the human Mac-1 antigen and is specific for the alpha chain.
The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.
Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human Mac-1 antigen; against CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human Mac-1 antigen; against CD11b
Comments
Animals were immunized with immunoprecipitates of human granulocyte lysates using TS1/18 (see ATCC HB-203) and boosted with live human granulocytes.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the human Mac-1 antigen and is specific for the alpha chain.
The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.
Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype mouse IgG1
Name of Depositor LJ Miller, TA Springer
Passage History
Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.
References

Miller LJ, et al. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation. J. Immunol. 137: 2891-2900, 1986. PubMed: 2428876

Zhang L, Plow EF. Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s):

References

Miller LJ, et al. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation. J. Immunol. 137: 2891-2900, 1986. PubMed: 2428876

Zhang L, Plow EF. Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418