GAD-1 (ATCC® HB-184)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with GAD in cells from chickens, mice, rats, rabbits, cats and macaque monkeys.
The enzyme GAD is present in and is a marker for neurons that use gamma amino butyric acid as a neurotransmitter.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glutamic acid decarboxylase (GAD)
Cellular Products
immunoglobulin; monoclonal antibody; against glutamic acid decarboxylase (GAD)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with GAD.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with GAD in cells from chickens, mice, rats, rabbits, cats and macaque monkeys.
The enzyme GAD is present in and is a marker for neurons that use gamma amino butyric acid as a neurotransmitter.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor DI Gottlieb
References

Gottlieb DI, et al. Monoclonal antibodies to glutamic acid decarboxylase. Proc. Natl. Acad. Sci. USA 83: 8808-8812, 1986. PubMed: 2430303

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gottlieb DI, et al. Monoclonal antibodies to glutamic acid decarboxylase. Proc. Natl. Acad. Sci. USA 83: 8808-8812, 1986. PubMed: 2430303