Y-3P (ATCC® HB-183)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k.
It cross-reacts with all rat I-A like molecules.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against I-A; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against I-A; cytotoxic
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k.
It cross-reacts with all rat I-A like molecules.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 10 mM HEPES, 95%; fetal bovine serum, 5%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor C Janeway
References

Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984. PubMed: 6228596

Basic Documentation
References

Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984. PubMed: 6228596