2.28 M1 [A2,28M1] (ATCC® HB-166)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic
Comments
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for a subpopulation of HLA A2 positive cells and HLA A28 positive cells at an appropriate titer.
The antibody recognizes an epitope at the amino terminal end of the alpha 2 domain of the HLA-A2 molecule.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgM
Name of Depositor F Grumet
References

Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666