Mouse hybridoma MAb 1812-2-4 (P450 1A2) (HB-12683)

Designation / Description Mouse hybridoma MAb 1812-2-4 (P450 1A2)
U.S. Patent Number
Biosafety Level 1

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Animals were immunized with baculovirus-expressed human cytochrome P450 1A2 [U.S. Pat. 6,335,428]. Spleen cells were fused ? MAb 26-7-5 (ATCC HB-12681), MAb 951-5-1 (ATCC HB-12684) and MAb 1812-4-8 (ATCC HB-12683) specifically bind to human cytochrome P450 1A2. They inhibit 1A2 enzyme activity [U.S. Pat. 6,335,428]. None of the three MAbs cross-react with human cytochromes 1A1, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, 3A4, and 3A5 as measured by ELISA and immunoblotting procedures. The antibodies exhibit strong immunoblotting to 1A2 [U.S. Pat. 6,335,428]. The antibodies can be used for screening drugs for metabolism by cytochrome P450 1A6, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population [U.S. Pat. 6,335,428].
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Basic Documentation

Gelboin HV, et al. Agents that bind to and inhibit human cytochrome P450 1A2. US Patent 6,335,428 dated Jan 1 2002

Yang TJ, et al. Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver. Pharmacogenetics 8: 375-382, 1998. PubMed: 9825829