MAb 1812-4-8 (ATCC® HB-12683)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); ?  (myeloma)

Tissue:  B lymphocyte; hybridoma

Applications
The antibodies can be used for screening drugs for metabolism by cytochrome P450 1A6, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population [U.S. Pat.
The antibodies exhibit strong immunoblotting to 1A2 [U.S. Pat.
Spleen cells were fused ? MAb 26-7-5 (ATCC HB-12681), MAb 951-5-1 (ATCC HB-12684) and MAb 1812-4-8 (ATCC HB-12683) specifically bind to human cytochrome P450 1A2. They inhibit 1A2 enzyme activity [U.S. Pat.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with baculovirus-expressed human cytochrome P450 1A2 [U.S. Pat. 6,335,428]. Spleen cells were fused ? MAb 26-7-5 (ATCC HB-12681), MAb 951-5-1 (ATCC HB-12684) and MAb 1812-4-8 (ATCC HB-12683) specifically bind to human cytochrome P450 1A2. They inhibit 1A2 enzyme activity [U.S. Pat. 6,335,428]. None of the three MAbs cross-react with human cytochromes 1A1, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, 3A4, and 3A5 as measured by ELISA and immunoblotting procedures. The antibodies exhibit strong immunoblotting to 1A2 [U.S. Pat. 6,335,428]. The antibodies can be used for screening drugs for metabolism by cytochrome P450 1A6, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population [U.S. Pat. 6,335,428].
Comments
Animals were immunized with baculovirus-expressed human cytochrome P450 1A2 [U.S. Pat. 6,335,428]. Spleen cells were fused ? MAb 26-7-5 (ATCC HB-12681), MAb 951-5-1 (ATCC HB-12684) and MAb 1812-4-8 (ATCC HB-12683) specifically bind to human cytochrome P450 1A2. They inhibit 1A2 enzyme activity [U.S. Pat. 6,335,428]. None of the three MAbs cross-react with human cytochromes 1A1, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, 3A4, and 3A5 as measured by ELISA and immunoblotting procedures. The antibodies exhibit strong immunoblotting to 1A2 [U.S. Pat. 6,335,428]. The antibodies can be used for screening drugs for metabolism by cytochrome P450 1A6, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population [U.S. Pat. 6,335,428].
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with 5% Origen Hybridoma Cloning Factor and 15% fetal bovine serum
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor The United States of America
References

Gelboin HV, et al. Agents that bind to and inhibit human cytochrome P450 1A2. US Patent 6,335,428 dated Jan 1 2002

Yang TJ, et al. Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver. Pharmacogenetics 8: 375-382, 1998. PubMed: 9825829

Basic Documentation
References

Gelboin HV, et al. Agents that bind to and inhibit human cytochrome P450 1A2. US Patent 6,335,428 dated Jan 1 2002

Yang TJ, et al. Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver. Pharmacogenetics 8: 375-382, 1998. PubMed: 9825829