Hybridoma, hCD40L-M91 [M91, hCD40L-M91] (ATCC® HB-12056)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c  (B cell); BALB/c (myeloma)

Applications
Spleen cells were fused with either Ag8.653 or NS1 mouse myeloma cells (the specific line used was not specified).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with either Ag8.653 or NS1 mouse myeloma cells (the specific line used was not specified).
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD40 ligand (CD154, CD40L)
Cellular Products
immunoglobulin; monoclonal antibody; against human CD40 ligand (CD154, CD40L)
Comments
Animals were immunized with human CD40 ligand (CD154, CD40L).
Spleen cells were fused with either Ag8.653 or NS1 mouse myeloma cells (the specific line used was not specified).
The antibody recognizes the human CD40L polypeptide.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 , IgG1
Name of Depositor Immunex Corporation
References

Armitage RJ, et al. Monoclonal antibodies to CD40 ligand, pharmaceutical composition comprising the same and hybridomas producing the same. US Patent 5,961,974 dated Oct 5 1999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Armitage RJ, et al. Monoclonal antibodies to CD40 ligand, pharmaceutical composition comprising the same and hybridomas producing the same. US Patent 5,961,974 dated Oct 5 1999