B505 (ATCC® HB-12000)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The B505 antibody specifically binds to hLH beta core fragment without cross-reacting with hLH, hLH beta or human chorionic gonadotropin (hCG) beta core fragment [U.S. Pat.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with human luteinizing hormone (hLH) beta core fragment isolated from human pituitary extracts. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The B505 antibody specifically binds to hLH beta core fragment without cross-reacting with hLH, hLH beta or human chorionic gonadotropin (hCG) beta core fragment [U.S. Pat. 5,976,876].
Genes Expressed
immunoglobulin; monoclonal antibody; against human luteinizing hormone (hLH) beta core fragment
Cellular Products
immunoglobulin; monoclonal antibody; against human luteinizing hormone (hLH) beta core fragment
Comments
Animals were immunized with human luteinizing hormone (hLH) beta core fragment isolated from human pituitary extracts. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The B505 antibody specifically binds to hLH beta core fragment without cross-reacting with hLH, hLH beta or human chorionic gonadotropin (hCG) beta core fragment [U.S. Pat. 5,976,876].
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Name of Depositor Trustees of Columbia University
References

Canfield RE, et al. Antibodies specific for hLH beta core fragment. US Patent 5,976,876 dated Nov 2 1999

Birkin S, et al. Determination of the amount of hLH.beta. core fragment in a sample from a subject and uses thereof. US Patent 6,521,416 dated Feb 18 2003

Basic Documentation
References

Canfield RE, et al. Antibodies specific for hLH beta core fragment. US Patent 5,976,876 dated Nov 2 1999

Birkin S, et al. Determination of the amount of hLH.beta. core fragment in a sample from a subject and uses thereof. US Patent 6,521,416 dated Feb 18 2003