5D5.11.6 [5D5] (ATCC^® HB-11895^™)

5D5.11.6 [5D5] ATCC^® HB-11895^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Strain	Strain: BALB/c (B cell); BALB/c (myeloma)
Storage Conditions	liquid nitrogen vapor phase

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Derivation	Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597).
Genes Expressed	immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor
Comments	Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml. Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C

Isotype	IgG1 kappa , IgG1; kappa light chain

Name of Depositor	Genetech, Inc.
References	Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997 Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997 Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002

Name of Depositor

Genetech, Inc.

References

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997

Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph
References	Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997 Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997 Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002