G2b.2 (ATCC® HB-109)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Cellular Products
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Comments
Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
This line is a switch variant of Genox 3.53 (ATCC HB-103).
An IgG2a switch variant of Genox 3.53 also exists (G2a.5, ATCC HB-110).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI medium 1640 with HT (containing hypoxanthine, 10(-4) M and
thymidine, 1.6 x 10(-5) M), 90%; fetal bovine serum, 10%. Cells do not do well
if hypoxanthine and thymidine are omitted.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor P Parham
References

Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068