G2b.2 (ATCC® HB-109)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Cellular Products
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Comments
Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
This line is a switch variant of Genox 3.53 (ATCC HB-103).
An IgG2a switch variant of Genox 3.53 also exists (G2a.5, ATCC HB-110).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI medium 1640 with HT (containing hypoxanthine, 10(-4) M and
thymidine, 1.6 x 10(-5) M), 90%; fetal bovine serum, 10%. Cells do not do well
if hypoxanthine and thymidine are omitted.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor P Parham
References

Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068