SH2 (ATCC® HB-10743)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  CB6F1/J (B cell); BALB/c (myeloma)

Derivation
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human mesenchymal stem cells
Cellular Products
immunoglobulin; monoclonal antibody; against human mesenchymal stem cells
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with human mesenchymal stem cells.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody with mesenchymal stem cells from bone marrow.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 20%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Isotype IgG1
    Name of Depositor Osiris Therapeutics, Inc.
    References

    Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996

    Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996

    Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137