7A9 (ATCC® HB-10135)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to IL-1 activated human endothelial cells, but not to resting endothelial cells or IL-1 treated mononuclear leukocytes, fibroblasts or keratinocytes.
Derivation
Spleen cells were fused with mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against interleukin 1 (IL-1) activated human endothelial cells
Cellular Products
immunoglobulin; monoclonal antibody; against interleukin 1 (IL-1) activated human endothelial cells
Comments
Animals were immunized with IL-1 activated human umbilical vein endothelial cells.
Spleen cells were fused with mouse myeloma cells.
The antibody binds to IL-1 activated human endothelial cells, but not to resting endothelial cells or IL-1 treated mononuclear leukocytes, fibroblasts or keratinocytes.
It precipitates an antigen consisting of a 100000 dalton polypeptide (90000 daltons under reducing conditions).
The antibody totally blocks binding of granulocytes and partially blocks monocyte binding to IL-1 stimulated endothelial cells.
Complete Growth Medium RPMI 1640 medium with hypoxanthine and thymidine, 85%; fetal bovine serum, 15%
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 0.016 mM thymidine, 0.1 mM hypoxanthine,
85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Otsuka Pharmaceuticals Co., Ltd.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Newman W, Wilson DO. Monoclonal antibodies directed to IL-1 activated endothelial cells and medicaments employing the monoclonal antibodies. US Patent 5,011,778 dated Apr 30 1991

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Newman W, Wilson DO. Monoclonal antibodies directed to IL-1 activated endothelial cells and medicaments employing the monoclonal antibodies. US Patent 5,011,778 dated Apr 30 1991