CMalphabeta1h [CMab1h] (ATCC® CRL-8401)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  /  Disease: Papilloma

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Papilloma
Gender female
Strain RIII
Applications
produces chorionic gonadotropin, beta polypeptide
produces glycoprotein hormones, alpha polypeptide chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha
Storage Conditions liquid nitrogen vapor phase
Derivation
The alpha hCG cDNA was isolated from placental tissue.
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter.
HeLa Markers N
Genes Expressed
glycoprotein hormones, alpha polypeptide(chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha) [CGA],chorionic gonadotropin, beta polypeptide, glycoprotein hormones, alpha polypeptide
Comments
The alpha hCG cDNA was isolated from placental tissue.
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter.
The cells produce very high levels of alpha and beta hCG.
The line contains the plasmids pRF 398 (beta hCG) and pRF 375 (alpha hCG).
pRF398 contains the entire sequence of beta hCG plus 30 bp 5' untranslated and 60 bp 3' untranslated, the mouse metallothionein promoter, and bovine papilloma virus sequences.
pRF375 contains the entire sequence of alpha hCG plus 10 bp 5' untranslated and 220 bp 3' untranslated, the mouse metallothionein promoter, and bovine papilloma virus seqeunces.
A BamHI fragment (beta hCG) of pbeta579 was inserted in the BglII site of CL28 to create pRF394. A BamHI/SalI fragment (beta hCG and MT-1 promoter) of pRF394 was ligated to a 7.9 kb BamHI/SalI fragment (BPV sequences) of pB2-2 to create pRF398.
A BamHI fragment (alpha hCG) of palpha574 was inserted in the BglII site of CL28 to create pRF302. A BamHI/SalI fragment (alpha hCG and MT-1 promoter) of pRF302 was ligated to a 7.9 kb BamHI/SalI fragment (BPV sequences) of pB2-2 to create pRF375.
Complete Growth Medium Dulbecco's modified Eagle's medium with 20 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
fetal bovine serum, 90%; glycerol, 10%
Name of Depositor Genzyme Corp.
References

Reddy VB, et al. Heteropolymeric protein. US Patent 4,840,896 dated Jun 20 1989

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reddy VB, et al. Heteropolymeric protein. US Patent 4,840,896 dated Jun 20 1989