MJ [G11] (ATCC® CRL-8294)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: peripheral blood  /  Disease: cutaneous T cell lymphoma; mycosis fungoides

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Retrovirus]
Disease cutaneous T cell lymphoma; mycosis fungoides
Age 50 years
Gender male
Ethnicity Caucasian
Derivation
This line was established by Popovic et al. in 1983 from peripheral blood of a patient with CTCL.
Clinical Data
male
50 years
Caucasian

Antigen Expression
CD2 +; CD3 +; CD4 +
Receptor Expression
interleukin 2 (IL-2)
Genes Expressed
HTLV-I,CD2 +; CD3 +; CD4 +
Cellular Products
HTLV-I
Comments
The cells produce Human T cell Leukemia virus (HTLV-I) and HTLV related antigens.

The cells express a cell surface glycoprotein of 61000 daltons (45000 dalton when not glycosylated) that is characteristic of HTLV infection.


Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing Pellet cells by centrifugation, save one third of the spent medium and to two volumes of fresh medium. Resuspend the cell pellet in the mixture and dispense into new flasks. Establish new cultures at about 1 X 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,14
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6,7
TPOX: 8,11
vWA: 15,17
Name of Depositor President and Fellows of Harvard College
Year of Origin 1983
References

Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988

Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988

Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519