FL74-UCD-1 (ATCC® CRL-8012)

Organism: Felis catus, cat  /  Cell Type: lymphoblast  /  Disease: Leukemia,Leukemia,Leukemia

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Organism Felis catus, cat
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease Leukemia,Leukemia,Leukemia
Applications
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
The cells are capable of producing large amounts of feline leukemia that has attenuated virulence for cats, but which is capable of being used for vaccine preparation.
Derivation
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
Genes Expressed
Feline leukemia virus (FeLV), KT-FeLV-UCD-1 strain
Comments
These cells are persistently infected with the KT-FeLV-UCD-1 feline leukemia virus.
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
The cells are capable of producing large amounts of feline leukemia that has attenuated virulence for cats, but which is capable of being used for vaccine preparation.
Complete Growth Medium Leibovitz's L-15 medium with 2mM L-glutamine, 85%; fetal bovine serum, 15%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation).


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor NC Pedersen
Passage History
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
References

Pedersen NC, Theilen GH. Vaccine for preventing persistent feline leukemia viremia in cats. US Patent 4,264,587 dated Apr 28 1981

Theilen GH, et al. Replication of cat leukemia virus in cell suspension cultures. Nature 222: 589-590, 1969. PubMed: 5818677

Pedersen NC, et al. Safety and efficacy studies of live- and killed-feline leukemia virus vaccines. Am. J. Vet. Res. 40: 1120-1126, 1979. PubMed: 230767

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Pedersen NC, Theilen GH. Vaccine for preventing persistent feline leukemia viremia in cats. US Patent 4,264,587 dated Apr 28 1981

Theilen GH, et al. Replication of cat leukemia virus in cell suspension cultures. Nature 222: 589-590, 1969. PubMed: 5818677

Pedersen NC, et al. Safety and efficacy studies of live- and killed-feline leukemia virus vaccines. Am. J. Vet. Res. 40: 1120-1126, 1979. PubMed: 230767