A101D (ATCC® CRL-7898™) Organism: Homo sapiens, human / General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Age 56 years Gender male Ethnicity Caucasian Applications This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Storage Conditions liquid nitrogen vapor phase Karyotype modal number = 63; range = 48 to 82 Images Derivation Part of the NBL Cell Line Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue Clinical Data ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissuemaleCaucasian56 years Comments Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: Remove and discard culture mediumBriefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains a trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subcultivation Ratio: A ratio of 1:4 is recommendedMedium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: culture medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°C STR Profile Amelogenin: XCSF1PO: 11D13S317: 11,12D16S539: 12D5S818: 12D7S820: 10THO1: 8,9.3TPOX: 8,11vWA: 16,18 Isoenzymes ADA, 1ES-D, 1G6PD, BPEP-D, 1PGD, APGM1, 1-2PGM3, 1 Passage History Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. References Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS Other Documentation Cell Micrograph References Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758